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Objective:To investigate the clinical features of patients with Langerhans cell histiocytosis (LCH), and analyze the association between BRAF V600E mutation status and clinical features. Methods:A retrospective analysis was carried out for the clinical data of 60 patients with LCH at the Department of Pediatric Oncology, Sun Yat-sen Memorial Hospital between April 2013 and December 2019.Among them, 39 patients undertook BRAF V600E mutation testing, which in paraffin-embedded tissue samples were detected by quantitative real-time PCR (qRT-PCR), and in peripheral blood and/or bone marrow were tested by high-throughput sequencing, for analyzing the correlation between BRAF V600E mutation and clinical characteristics of LCH. Results:(1)Clinical characteristics: the age of 60 LCH patients was (4.08±0.45) years, with 43 male cases and 17 female cases.Patients at young age (≤2 years) and with risk organ (RO+ ) and central nervous system (CNS) risk lesions involvement were concentrated in the multisystem involvement (MS) group ( P<0.05). (2)Therapeutic response after induction therapy: the response to induction therapy was achieved in 28 of 60 treated patients (41.7%) and 32 (53.3%) did not.After excluding stratification factors of treatment regimen, MS ( OR=6.855, 95% CI: 2.077-22.622, P=0.002) and the age≤2 years ( OR=4.944; 95% CI: 1.601-15.275; P=0.005) were risk factors in poor chemotherapy response.RO+ ( OR=8.250, 95% CI: 1.617-42.090, P=0.005) was a significant risk factor for a poor chemotherapy response in JLSG-02 treatment group.Differently, RO+ had no dramatic effect on chemotherapy response in CCHG-LCH-2019 treatment group.(3) BRAF V600E mutation: 39 patients were determined BRAF V600E status, with the positive rate of BRAF V600E mutation in paraffin-embedded tissue samples reaching 70.3%(26 cases). BRAF V600E mutation was not associated with early treatment response, age, sex, MS and RO+ ( P>0.05). However, the positive rate of BRAF V600E in children with MS and CNS risk lesions was higher than the controls, with 76.0% (19 cases) vs.57.1% (8 cases) and 74.1% (20 cases) vs.58.3% (7 cases), respectively.Totally, 3 of 8 cases were positive in bone marrow, with 2 cases of MS, and 1 case of multiple bone invasions, and 1 of 5 cases was positive in peripheral blood, with liver and spleen being involved. Conclusions:LCH patients with age≤2 years, MS and RO+ exhibited a poor response to initial treatment, required for more aggressive treatment strategy.Lesion with activating BRAF V600E mutations suggests that LCH is a clonal disorder.There may be great variability between BRAF V600E mutations and MS as well as CNS risk lesions.In the mutation dataset, part of patients had positive BRAF V600E mutations in bone marrow/peripheral blood.This might suggest a different pathogenesis in such patients, has a certain clinical sense in some aspect.
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OBJECTIVE:To provide reference for ensuring safe and effective drug use in obstetrics and gynecology outpatient department.METHODS:Medication education intervention was conducted among some patients in obstetrics and gynecology outpatient department from 4 third grade class A hospitals of our province through making Wechat pushing messages,videos and leaflets.The difference of rational drug use knowledge awareness and compliance was compared before and after intervention by questionnaire survey.RESULTS:A total of 60 questionnaires were distributed,and 60 valid questionnaires were collected with effective recovery rate of 100％.Compared to before intervention,correct rate of 20 questions about the knowledge of rational drug use were improved after intervention in respects of awareness and compliance.The awareness and compliance scores about the knowledge of rational drug use after intervention were higher than before intervention;there was statistical significance in Wechat pushing message group [(53.18 ± 11.51) vs.(88.48 ± 7.12),(55.15 ± 11.82)vs.(86.81 ± 7.69)],in video group [(49.50 ± 17.23) vs.(85.00 ± 11.55),(52.00 ± 17.70)vs.(86.00 ± 6.99)],in leaflets group[(41.47 ± 9.14)vs.(77.05 ± 9.36),(43.23 ± 10.89)vs.(78.82 ± 9.11)] be-fore and after intervention (P＜0.05).There was no statistical significance in the improvement of awareness or compliance score among those groups (P=0.992 and P=0.397).CONCLUSIONS:Three intervention methods can effectively improve the awareness and compliance of patients about rational drug use knowledge in obstetrics and gynecology outpatient department.Pharmacists can choose the appropriate medication education intervention based on the patient's different educational levels,preferences and acceptability.
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OBJECTIVE:To provide reference for ensuring safe and effective drug use in obstetrics and gynecology outpatient department.METHODS:Medication education intervention was conducted among some patients in obstetrics and gynecology outpatient department from 4 third grade class A hospitals of our province through making Wechat pushing messages,videos and leaflets.The difference of rational drug use knowledge awareness and compliance was compared before and after intervention by questionnaire survey.RESULTS:A total of 60 questionnaires were distributed,and 60 valid questionnaires were collected with effective recovery rate of 100％.Compared to before intervention,correct rate of 20 questions about the knowledge of rational drug use were improved after intervention in respects of awareness and compliance.The awareness and compliance scores about the knowledge of rational drug use after intervention were higher than before intervention;there was statistical significance in Wechat pushing message group [(53.18 ± 11.51) vs.(88.48 ± 7.12),(55.15 ± 11.82)vs.(86.81 ± 7.69)],in video group [(49.50 ± 17.23) vs.(85.00 ± 11.55),(52.00 ± 17.70)vs.(86.00 ± 6.99)],in leaflets group[(41.47 ± 9.14)vs.(77.05 ± 9.36),(43.23 ± 10.89)vs.(78.82 ± 9.11)] be-fore and after intervention (P＜0.05).There was no statistical significance in the improvement of awareness or compliance score among those groups (P=0.992 and P=0.397).CONCLUSIONS:Three intervention methods can effectively improve the awareness and compliance of patients about rational drug use knowledge in obstetrics and gynecology outpatient department.Pharmacists can choose the appropriate medication education intervention based on the patient's different educational levels,preferences and acceptability.
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Objective To investigate PSP on bone microstructures,Ca,P,OPG and RANKL of osteoporotic rat model.Methods Thirty female rats randomly divided into five groups:Sham,OVX,H-,M-,L-PSP.Sham and OVX were irrigated stomachsaline;PSP solution was gavaged to other groups.After 8-week,bone microstructures of tibial metaphyseal,Ca,P,OPG and RANKL were measured.Results Body weight,Ca,P,RANKL,Tb.Sp of OVX were significantly increased compared to Sham,OPG,BV/TV,Tb.Th,Tb.N decreased.Body weight of H-,M-PSP,Ca and Tb.Sp of PSP,P and RANKL in H-PSP were decreased compared to OVX,OPG in H-,M-PSP,BV/TV,Tb.Th,Tb.N of PSP group increased.The differences were statistically significant (P ＜ 0.05).Conclusion PSP prevents osteoporosis by improving the microstructure of trabecular bone,reducing bone turnover,increasing OPG and reducing RANKL expression.
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OBJECTIVE:To investigate the effects of simvastatin intensive treatment on the Postoperative Related Indexes of patients with acute coronary syndrome(ACS) underwent percutaneous coronary intervention(PCI). METHODS:106 patients with were included in the study and randomly divided into observation group(53 cases)and control group(53 cases). Both groups were given aspirin 100 mg,qd+clopidogrel 75 mg,qd before PCI for 4 weeks;observation group was additionally given Simvastatin tablet orally 20 mg before supper 15 d before surgery. TC,TG,LDL-C,HDL-C,hs-CRP,IL-6 and IL-18 levels,LVEF,the occurrence of coronary artery restenosis were detected in 2 groups before surgery and 6 months after surgery. The occurrence of ADR was recorded during treatment. RESULTS:There was no statistical significance in the levels of TG,TC,LDL-C and HDL-C between 2 groups before surgery and 6 months after surgery (P>0.05). There was no statistical significance in hs-CRP,IL-18, IL-6 and LVEF levels between 2 groups before surgery(P>0.05). 6 months after surgery,hs-CRP,IL-6,IL-18 and LVEF levels of 2 groups were significantly higher than before treatment;hs-CRP,IL-6 and IL-18 levels of observation group were significantly lower than those of control group,and LVEF was significantly higher than control group,with statistical significance (P<0.05). The incidence of coronary artery restenosis in observation group was significantly lower than control group, with statistical significance (P<0.05). No ADR was found in 2 groups during treatment. CONCLUSIONS:Preoperative simvastatin intensive treatment can effectively reduce cardiovascular inflammation degree in patients with ACS after PCI,prevent the formation of coronary artery thrombus,and reduce the incidence of coronary artery restenosis so as to effectively improve the prognosis and don' t increase the incidence of ADR.
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OBJECTIVE:To explore loading dose simvastatin on related indicators in PCI perioperative period of patients with acute myocardial infarction. METHODS:Data of 203 acute myocardial infarction patients undergoing emergency PCI were retro-spectively collected and divided into observation group (102 cases) and control group (101 cases) by different regimens. Control group received conventional treatment for 3 d before PCI,including orally taking Aspirin enteric-coated tablet 300 mg/d,qd + Sim-vastatin tablet 40 mg/d,qd,simvastatin 40 mg/d after surgery,qd,for 4 weeks. Observation group received Simvastatin tablet 80mg 2 h before PCI,the other treatment was the same with control group. Total cholesterol(TC),triglyceride(TG),low-density li-poprotein cholesterol (LDL-C),high-density lipoprotein cholesterol(HDL-C),IL-6,IL-10,TFN-α,plasma super-sensitive tropo-nin(TNT-HSST)level,creatine kinase isoenzyme(CKMB)level,high sensitive C-reactive protein(hs-CRP)level and transami-nase level before and 24 h after treatment were observed and the incidence of adverse reactions was recorded. Meanwhile,the inci-dence of postoperative 30 d of MACE and CIN was followed-up. RESULTS:There was no significant difference in TG,TC, LDL-C,HDL-C before and after treatment(P>0.05). Before treatment,there was no significant difference in TNF-α,IL-6,IL-10, hs-CRP,CKMB,TNT-HSST levels in 2 groups (P>0.05);after treatment,TNF-α,IL-6,hs-CRP,CKMB,TNT-HSST levels were significantly higher than before(P<0.05),and TNF-α,IL-6,hs-CRP levels in observation group was significantly lower than control group,CKMB,TNT-HSST levels were significantly higher than control group;IL-10 was significantly lower than before in 2 groups,and observation group was higher than control group,with statistical significance (P<0.05). The MACE rate and CIN rate in observation were lower than control group with statistical significance(P<0.05). And no obvious adverse reaction was found in 2 groups. CONCLUSIONS:Loading dose simvastatin in PCI perioperative period can significantly reduce patients' PCI, TNF-α,hs-CRP,CKMB,TNT-HSST levels and the incidence of cardiovascular and renal adverse reactions.
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Objective:To analyze PD-L2 expression on monocytes of peripheral blood cells in systemic lupus erythematosus ( SLE) and it′s correlation with the degree of disease activity .Methods:Peripheral blood of 26 cases of SLE patiens and 38 cases of healthy controls were collected .Peripheral blood mononuclear cells ( PBMC) were isolated and realtime PCR was carried on to analyze the PD-L2 gene expression.At the same time flow cytometry was performed to analyze the CD 14 and PD-L2 expression.Results:PD-L2 was significantly up-regulated on monocytes in RA patients than in healthy controls and had correlation with the disease activity and the SLEAI score.Conclusion:These findings help to clarify the function of PD-L2,including its potential role as a biomarker for SLE .
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@#To investigate the effect of block of AGEs-RAGE pathway on the migration of aortic vascular smooth muscle in diabetic rats and its possible mechanisms, vascular smooth muscle cells(VSMCs)cells were pre-stimulated by antibody of RAGE, and then stimulated by AGEs. Transwell assay was adopted to assay migration of VSMCs. Proliferation of VSMCs and expression of p27 were analyzed by MTT and ELISA, respectively. The change of ROS level in VSMCs was defermined by DCFH assay, the expression of NOX1 mRNA was determined by RT-PCR assay. Results indicated that the AGEs induction for migration of VSMCs was significantly inhibited after treatment by RAGE antibody(P< 0. 01), which blocked the AGEs-RAGE pathway, and the inhibition of migration was stronger than that of proliferation. The ROS level was decreased(P< 0. 01), and the expression of NOX1 mRNA was decreased, yet the expression of P27 protein was not changed greatly. Block of AGEs-RAGE pathway by antibody of RAGE can inhibit the migration of VSMCs, and the mechanism may be related with the decrease of NOX1 mRNA and then down to the level of intracellular oxidative stress in VSMCs.
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AIM: To investigate the effects of human umbilical cord-derived mesenchymal stem cells ( hUC-MSCs) on the proliferation and migration of osteosarcoma cells ( Saos-2 ) and the underlying molecular mechanism. METHODS:hUC-MSCs were isolated and cultured by tissue explants adherent method.The cell surface markers on hUC-MSCs were identified by flow cytometry.The effects of conditioned medium ( CM) from hUC-MSCs ( hUC-MSCs-CM) , re-combinant human interleukin-6 (rhIL-6) and IL-6 neutralizing antibody on the proliferation of Saos-2 cells were detected by CCK-8 assay and cell counting.IL-6 secretion of hUC-MSCs was assayed by ELISA.RT-PCR was used to assess the tran-scription level of proliferation-related genes proliferating cell nuclear antigen ( PCNA) , cyclin D1 and survivin.The migra-tion potential of hUC-MSCs and Saos-2 cells was measured by Transwell assay.RESULTS:hUC-MSCs migrated to Saos-2 cells.hUC-MSCs-CM contained a high concentration of IL-6, up to (1 835.5 ±134.1) ng/L.hUC-MSCs-CM and rhIL-6 promoted the proliferation and migration of Saos-2 cells.Addition of neutralizing antibody against IL-6 in the hUC-MSCs-CM impaired this proliferation and migration of Saos-2 cells.The mRNA expression of PCNA, cyclin D1 and survivin was up-regulated by hUC-MSCs-CM and rhIL-6, while this effect was dramatically attenuated by treatment with IL-6 neutralizing antibody.CONCLUSION:hUC-MSCs migrate to osteosarcoma cells and promote the proliferation and migration of osteo-sarcoma cells through secreting IL-6 in vitro.
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Objective Previous study found TGF-βpathway might be the molecular pathway influencing the prognosis of colo-rectal cancer, while it was uncertain whether Chinese population is associated with the disease.The article was to evaluate the genetic factors associated with prognosis in colorectal cancer. Methods 52 cases patients with colorectal cancer were followed-up for 36 months in our hospitals from January 2013 to August 2014.Their DNAs were extracted and stored and gene typing were carried out in 5 candidate genes to detect the association between SNPs and the prognosis in colorectal cancer. Results The results showed that within the TGF-βsignaling pathway, after adjusting for Bonferroni multiple testing, allele A of SNP rs10749971 located in gene POU2AF1 was associated with the recurrence of patients with stage III disease under additive and recessive genetic models ( HR =1.968, P=0.004;HR=2.174, P=0.010).Allele C of SNP rs961253 in the gene BMP2 could increase the recurrence risk (HR=1.992, P=0.005) and the death risk (HR=3.161, P=0.007) of patients with stage III disease under recessive genetic models.Allele A of SNP rs4464148 in SMAD7 gene could significantly decrease the death risk of patients with stage II and III colorectal cancer under dominant genetic model (HR=0.382, P=0.017;HR=0.230, P=0.006).In addition, accumulated effects of several adverse genes showed gene high risk group could increase the risk of death for patients with stage III colorectal cancer significantly ( HR=15.512, P=0.036;95%CI:1.611-149.360). Conclusion In different genetic models, SNP locus mutation within gene POU2AF1, BMP2 and SMAD7 on TGF-βpathway was associated with the prognosis of patients with colorectal cancer.With the increase of the number of unfavorable genes, the death risk increases accordingly.
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Objective To compare the difference of ELISA and LABScreen in detecting HLA antibodies and evaluate their effects on allograft rejection.Methods Consecutive patients undergoing kidney transplantion from November,2008 to December,2009 in the First Affiliated Hospital and the following up patients during the same period,with abnormal Scr who had completed kidney biopsies,were included in this study.Patients' HLA antibodies were detected by ELISA (Lambda antigen tray,LATTM) and LABScreen Mix Beads (LABScreen MIX,One Lambda,Canoga Park,CA,USA) or LABScreen Single antigen beads (LABScreenTM single antigen beads,One Lambda,Canoga Park,CA,USA).Patients' Scr were also detected at different time potints.Results There were 277 patietns included.Among them 145 (52.3％) cases were HLA antibody positive detected by LABScreen,which including 118 cases ELISA negative but LABScreen positive,and 27 cases both ELISA and LABScreen positive.No case was ELISA positive but LABScreen negative.Among 118 cases which were LABScreen positive but ELISA negative,41 (34.7％) cases happened acute or chronic rejection.However,only 24 cases happened rejection in 132 double negative cases (18.2％,P =0.003).There were 31％ patients in rejection group while only 12.8％ patients (P=0.01) in non-rejection group whose HLA antibody fluorescence intensity detected by LABScreen single antigen beads still increased two weeks after transplantation.Conclusion LABScreen is more sensitive than ELISA in detecting HLA antibodies,and its result highly correlates with the incidence of allograft rejection.
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Teaching medical courses in French are the characteristics of teaching programs in medicine school of Shanghai JiaoTong uuiversity.It may help develop the course content and promote the international academic communication.Teaching pathology course in French for Chinese students is also an important task of pathology teaching program.The paper introduced and explored the language use of French,regional variations between Chinese and French pathological major and the cooperation with foreign professors in teaching.It is been testified that teaching in French can contribute to the medical education and arouse student's learning initiative.
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Objective To study the role of deleted in liver cancer-1 (DLC-1) gene main domains on the regulation of human colon cancer HT29 cell proliferation.Methods Subcloning recombinant plasmid vectors with Rho GTPase activating protein (RhoGAP),sterile alpha motif (SAM) or steroidogenic acute regulatory-related lipid-transfer (START) domains of DLC-1 gene knockout were constructed and transfected into human colon cancer cell HT29.Wild HT29 cell group (control group),pcDNA3.1-HT29 cell group (vector group) and pcDNA3.1-HT29-DLC-1 cell group (whole DLC-1 gene transfected group) were set as control.The change of cell proliferation was detected by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and colony formation test.The cell apoptosis was analyzed by flow cytometry.The activity of RhoA protein was detected by pull-down assay.The differences between the groups were analyzed by the analysis of variance.Results At 48 hours after the successful transfection,compared with control group and vector group,cells proliferation and the activity of RhoA protein were significantly suppressed in whole DLC-1 gene transfected group (F=146.36,698.08,both P＜0.05) and early cell apoptosis increased (F=294.08,P＜0.05).Compared with control group and vector group,there was no significant difference in cell proliferation ability,cell apoptosis and the activity of RhoA protein activity in RhoGAP knockout transfected cells (F=0.99,0.049,5.769,all P＞0.05).Compared with whole DLC-1 gene transfected group,the suppression of cell proliferation was more significant in SAM knockout transfected cells (F=31.00,P＜0.05),the activity of RhoA protein down regulated (F=92.57,P＜0.05) and apoptosis increased (F=130.44,P＜0.05).Compared with whole DLC-1 gene transfected group,the ability of cell proliferation increased (F=15.47,P＜0.05),apoptosis cell decreased (F＝110.23,P＜0.05) and the activity of RhoA protein up regulated (F=199.39,P＜0.05) in START knockout transfected cells.Conclusions The role of DLC-1 gene in the suppression of cell proliferation in HT29 cells was RhoGAP-dependent.SAM domain may be the self suppression domain for endogenous RhoGAP activity.START domain may take effect through enhancing RhoGAP domain.
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Aim: To observe the effect of ursolic acid(UA)on the proliferation of rat vascular smooth muscle cell(VSMC)induced by high-level glucose and explore its relationship with p38MAPK signal transduction pathway.Methods: The proliferation of VSMC induced by high-level glucose(25 mmol/L glucose)was adopted as model,the inhibition of UA on the proliferation of VSMC was measured by MTT assay,and the expression lev-els of phospho-p38MAPK was detected by cell-based ELISA as well as the expression of c-fos protein was exam-ined by SABC method.Results: UA(20 μmol/L and 40 μmoL/L)inhibited glucose-induced proliferation of VSMC(P <0.05).Compared with the group subjected to glucose induction,UA decreased the expression levels of phosphorylated p38MAPK(P < 0.05),and also inhibited c-fos expression.Conclusion: UA suppressed glucose induced proliferation of VSMC,which might be related to the suppression of the activation of p38MAPK signal transduction pathway,and thereby down-regulated c-fos expression.
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Objective To study the relationship between the expression of human Stanniocalcin-1(hSTC-1) mRNA in the peripheral blood from patients with colorectal cancer and its malignant behavior. Methods RT-PCR was used to detect hSTC-1 mRNA in the peripheral blood from 57 patients with colorectal cancer. The peripheral blood from 14 patients with gastrointestinal inflammatory diseases, 15 healthy volunteers and 5 pregnant women were served as controls. Results The positive rate of hSTC-1 mRNA in 57 patients with colorectal cancer was 49.12% (28/57), and the mRNA expression of hSTC-1 was significantly related with the clinical stage of colon cancer (P